This morning session, chaired by Ann Knolhoff of the Food and Drug Administration, starts at 8:30 am and will cover different approaches for developing and implementing non-targeted and suspect screening workflows using high-resolution mass spectrometry to identify unknowns.
John Stephens of Bidwell Training Center (Pittsburgh, Pennsylvania) presides over this session, to be held in Room 118B and starting at 8:30 am.
This symposium on drug discovery is organized by Sammy Datwani, Room 117 from 1:30 pm–4:45pm.
In the late “noughties” we couldn’t avoid the webinars, seminars and online calculators which were being released by HPLC column manufacturers to extol the virtues of the “new” core-shell particle morphology that promised high performance at lower back pressures.
Click the title above to open The Column March 2019 North American issue, Volume 15, Number 3, in an interactive PDF format.
Click the title above to open The Column March 2019 Europe & Asia issue, Volume 15, Number 3, in an interactive PDF format.
This article describes how to prevent the loss of sample analytes by understanding the factors responsible for poor recoveries.
Agilent Technologies has awarded Paul Bonnington and Kimbal Marriott an Agilent Thought Leader Award in support of their innovative research into data analytics, machine learning, artificial intelligence, and information visualization.
Researchers from the Medical University of Lublin, Poland, have investigated the presence of KYNA in alcoholic beverages and the significance of its presence using HPLC.
Researchers from the University of Washington, USA, have developed an ultrafast separation using pulse flow valve modulation to enable high peak capacity in GC×GC and GC×GC×GC.
Knauer has been ranked as one of the top employers in Germany for 2019, according to a study by 3.works GmbH, a Düsseldorf-based employment research institute.
Virtually exclusively, liquid chromatography–mass spectrometry (LC–MS)-based assays for protein quantitation rely on bottom-up strategies, where the protein is initially digested into constituent peptides during sample preparation. Top-down intact protein quantitation, especially using affordable, low resolution triple quadrupole (QQQ) mass spectrometers, has been largely unexplored.
Click the title above to open the LCGC Asia Pacific March 2019 regular issue, Vol 22, No 1, in an interactive PDF format.
The offerings of commercially available columns for reversed-phase liquid chromatography (LC) continue to expand. Are these columns similar or different compared to what is already available?
Click the title above to open the Chromatography and Sample Preparation Terminology Guide, Vol 32, No s3, in an interactive PDF format.
Solid-phase microextraction (SPME) was introduced nearly 30 years ago and since that time has matured into a widely used tool in the arsenal of sample preparation techniques. Simultaneously, it has spawned a host of related techniques where sorbent coatings are placed on stir bars, magnetic particles, vial walls, and so on. Over the past few years, several advances in SPME have been developed, including increasing the sorbent surface area available for extraction, accommodating direct analysis by mass spectrometry (MS), and sorbent overcoating to resist fouling by sugars, lipids, and other macromolecules present in some sample types. These advances are discussed in this month’s instalment. The use of SPME for microsampling of biological systems, so-called bio-SPME, will be the focus of Part 2.
Click the title above to open the LCGC Europe March 2019 regular issue, Vol 32, No 03, in an interactive PDF format.
One millimetre internal diameter liquid chromatography columns are available from many manufacturers. In this article, the utility of 1.0-mm internal diameter (i.d.) columns, and the arenas in which they play a relatively strong role, are investigated. Further, the advantages and disadvantages of 1.0 mm diameter columns are contrasted with both larger- and smaller-bore formats.
The 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2019) will be held 16–20 June 2019 at the Milano-Bicocca University, Milan, Italy. This is the first time that this symposium will be held in Italy.
In the present study, a novel GC–MS TQ method was developed and validated for the simultaneous analysis of 2-MCPD, 3-MCPD, and glycidyl fatty acid esters in edible oil. This method was subsequently applied to quantitation of these contaminants in commercial edible oil samples.
In this study, we aimed to use a charge modulated HILIC stationary phase (iHILIC®-Fusion) to separate different siderophores of the Pseudomonas taiwanensis VLB120 bacteria.
This application note outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood. UCT’s Clean Screen® XCEL I column provides users with the same level of sample clean-up as traditional SPE while allowing the elimination of timely conditioning and wash steps.
For your highly sensitive UHPLC–MS analyses, how can you reduce noise and additional signals to a minimum?
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial mechanism of action (MoA) of anti-tumour therapeutic antibodies and FcγIIIa receptor plays a key role in this process by interacting with the N-glycans of IgG Fc regions. Hence, affinity chromatography on Fc receptor ligands can deliver valuable information about expected ADCC activity and mAb glycoform distribution.
This application note shows how to achieve robust chromatographic results for two commercially available mAbs: Adalimumab (Humira®) and Bevacizumab (Avastin®).
Branching affects macroscopic polymer properties such as crystallinity, melting temperature, toughness, ductility, and optical clarity. Two types of branching are long-chain branching (LCB) and short-chain branching (SCB), wherein the molar mass of the branches is larger or smaller than the entanglement molar mass, respectively.
Click the title above to open the LCGC North America March 2019 issue, Volume 37, Number 3, in an interactive PDF format.