Liquid Chromatography (LC/HPLC)

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.

Our annual review of new high performance liquid chromatography instruments, mass spectrometry systems, and data processing software.

Two-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.

With kinetic plots, you can make better-informed column choices. Here’s how.

A little preparation eases the challenges of new methods—and increases the fun.

This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.

Barry L Karger is Director Emeritus of the Barnett Institute, and is the James L. Waters Emeritus Chair and Distinguished Professor Emeritus at Northeastern University in Boston.

James P. Grinias is an Associate Professor in the Department of Chemistry & Biochemistry at Rowan University in Glassboro, New Jersey, and the winner of the 2022 Emerging Leader in Chromatography Award, which is presented by LCGC magazine.

A kinetic plot is a powerful tool, but how do you construct one—from either experimental data or data from other sources? We explain.

New developments in metal-organic frameworks (MOFs) for LC seek to control MOF distribution on the silica surface, improve understanding of the role of the substrate, and enhance robustness and reproducibility.

HPLC is emerging as the preferred analytical method for potency testing of cannabinoids, but fundamental work is needed to solve critical issues and contribute to advancing knowledge.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.

The presence of per- and polyfluoroalkyl substances (PFAS) in products used every day by millions of people is a cause of concern among both consumers and scientists. PFAS found in drinking water and the environment can cause serious health issues in animals and humans. Amanda Belunis, who is a PhD candidate at the University of Maryland in Baltimore County, has been investigating the use of liquid chromatography­­–tandem mass spectrometry (LC–MS/MS) to detect PFAS from a variety of environmental sources. She spoke with us about methods used to detect PFAS and described a new method that she and her team have developed to enhance PFAS detection.

Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.

QuEChERS has been updated to suit modern instrumentation. Now also “efficient and robust,” QuEChERSER is a “mega-method” that covers a wider polarity range.

We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.

Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.

Errors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.

Are we ready to let software and instruments take over the art of separations?

Here, a new automated active pharmaceutical ingredient (API) extraction platform is integrated with online liquid chromatography (LC) to assess drug product potency. The platform dissolves the tablet coating (if present), disintegrates the tablet, and disperses and solubilizes the tablet for analysis.

Deviations from the expected pressure in modern LC systems (too low, too high, or fluctuating) can be diagnosed and more quickly resolved using the streamlined troubleshooting practices shown here.

In this month’s blog, we provide information about the Subdivision on Chromatography and Separations Chemistry (SCSC) of the Analytical Division of the American Chemical Society (ACS), sharing our main goals and introducing our newly appointed executive board members.

An improved LC–MS/MS method that can rapidly detect fipronil, an insecticide harmful to human health, is presented here for chicken eggs, feed, and soil.