Liquid Chromatography (LC/HPLC)

Using Earth as a figurative analogy, we explore the merits of flexibility when considering which liquid chromatography (LC) modality to use for any particular bioanalysis.

We explain several of the more frequent causes of bad peak shapes in liquid chromatography and provide tips on how to remedy them.

Knowing the variables that most affect the sample loading capacity in a porous layer open tubular (PLOT) column is vital to determining how much sample to inject.

We kick off a new article series that offers an insider’s view of the pharmaceutical industry, focusing on the role of separation science and separation scientists.

In this issue, we feature fundamental and innovative efforts from within the walls of some the leading companies in the separation science industry. The theme was inspired by the continuous output of essential separation tools and the seemingly endless streams of pertinent information that these companies provide the scientific community.

Sustainability concerns are renewing interest in SFC, often as a substitute for HPLC. With the broader application of SFC, we need a better understanding of selectivity in SFC, where stationary phase classification is not well established. This study assessed and measured the selectivity differences afforded by three prototype SFC phases.

Superficially porous particles (SPPs) are routinely used in analytical liquid chromatography (LC) columns, but they also provide benefits for preparative LC columns for use in pharmaceutical discovery laboratories and high-throughput environments. In this study, a preparative method using SPP-packed column had a 45% decrease in run time over a method using columns packed with fully porous particles, at standard flow.

A novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.

Extraction-based sample preparation has been recognized as a significant step in separation science. To improve the sensitivity and selectivity of the extraction, some new approaches have been introduced, including those that employ an electric field. These electric field-assisted extraction methods create another dimension for sample preparation, and are compatible with miniaturized, portable, and multifunctional analytical platforms.

Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D-LC) separations. Here, two approaches are presented that can help navigate this challenge.

Our yearly report on liquid chromatography (LC) columns and accessories commercially released over the past year.

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.

Our annual review of new high performance liquid chromatography instruments, mass spectrometry systems, and data processing software.

Two-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.

With kinetic plots, you can make better-informed column choices. Here’s how.

A little preparation eases the challenges of new methods—and increases the fun.

This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.

Barry L Karger is Director Emeritus of the Barnett Institute, and is the James L. Waters Emeritus Chair and Distinguished Professor Emeritus at Northeastern University in Boston.

James P. Grinias is an Associate Professor in the Department of Chemistry & Biochemistry at Rowan University in Glassboro, New Jersey, and the winner of the 2022 Emerging Leader in Chromatography Award, which is presented by LCGC magazine.

A kinetic plot is a powerful tool, but how do you construct one—from either experimental data or data from other sources? We explain.

New developments in metal-organic frameworks (MOFs) for LC seek to control MOF distribution on the silica surface, improve understanding of the role of the substrate, and enhance robustness and reproducibility.

HPLC is emerging as the preferred analytical method for potency testing of cannabinoids, but fundamental work is needed to solve critical issues and contribute to advancing knowledge.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.