Liquid Chromatography (LC/HPLC)

Using an Analytical Quality by Design approach, scientists recently identified impurities in a pharmaceutical product for hypertension treatment.

Using liquid chromatography-tandem mass spectrometry, scientists have found a similarity between the protective functions of the oral permeability barrier and the skin barrier.

While pesticides may be beneficial, they may also cause health risks in foods. In a recent study, scientists created new methods for detecting maximum pesticide residue levels.

Dipeptide profiles have an important role in detecting disease, and a recent study has made it much more accessible to study them.

A new buffer management system that automates most processes will further how biopharmaceuticals are produced, helping save more lives in the process.

A recent study shows how 2D-LC–MS can provide expanded analytical coverage of cosmetics-prohibited substances.

By performing tissue analysis of caracals in captivity and in their natural African habitats, scientists were able to quantify uptake of toxic chemicals from the environment depending on how polluted they are.

A recent study was conducted using liquid chromatography–tandem mass spectrometry (LC–MS/MS) to identify the effect of cooking and storage on poly)phenolic compounds in piquillo peppers.

Online SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.

The variables that are most important for improving the separation of complex samples are quite different from those for simpler samples. Considering these differences can save time and resources.

This comparison of various silica-based HILIC stationary phases with different types of coatings provides a better understanding of how stationary phase selectivity can be influenced.

When the unique shape-selective properties of PGC are appropriately identified, PGC stationary phases can be harnessed for fine separations of structurally related compounds.

An examination of the new high performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products that were introduced over the last year.

LCGC Europe spoke to Giorgia Purcaro from the University of Liège in Belgium, about her work investigating mineral oil hydrocarbons (MOH) using GCxGC and the practical advantages that a novel LC–GCxGC–TOF-MS/FID method offers the analyst.

Serial coupling of different column types can provide several important benefits to the chromatographer, including fine-tuning selectivity to separate complex mixtures.

The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.

Pressure-enhanced liquid chromatography (PE-LC) offers a new approach for improving selectivity for large molecule separations. Examples shown here include short oligonucleotides in ion-pairing reversed-phase (IP-RP) liquid chromatography and larger nucleic acids in ion-exchange (IEX) chromatography.

A systematic approach to method improvement can save time and resources by using methods that are likely to be better, and more robust, than those developed using a trial-and-error approach.

The 2nd "Advances in Separation Science: From Extraction to Chromatographic Applications" workshop will be held from 28–29 June 2023 in the University of Liège in Belgium.

A novel LC–MS/MS method, with a dual ESI and APCI source using simple solvent extraction for sample preparation, was validated for analysis of 102 pesticides and 5 mycotoxins regulated by Colorado state in dried hemp.

Luis A. Colón is 2023's recipient of the Chromatography Forum of Delaware Valley Dal Nogare Award, which recognizes contributions to chromatographic science. The symposium took place at Pittcon 2023, which was held from March 18–22.

This article presents a new column technology that uses reversed phase separation and offers excellent peak shape, resolution, and robustness for a wide variety of food matrices.

When can analyte retention deviate from what is expected or normal? We explain three subtle causes.

On a fundamental level, equilibrium and diffusion define chemical separations. Practical applications of partition coefficients to chromatographic sample preparation illustrate this point.

A new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to assess drug product potency is presented.