HPLC

An overview of modern approaches to small-molecule drug discovery.

Sample preparation and analysis of pharmaceuticals in wastewater present unique challenges. Here, we describe those challenges.

Although smaller advances have been made in the past decades, the question remains whether further extending operating pressure and decreasing particle size remains a feasible approach, or whether drastically novel approaches are required.

The fundamental mechanisms of band broadening are usually introduced to students through the van Deemter equation. Dimensional analysis of this equation can give physical meaning to the equation coefficients and enhance our understanding relative to qualitative descriptions. This approach can also guide improvements to future liquid chromatography (LC) column designs.

In this article, I share my perspective on the trends in 2D-LC, and the developments we are likely to see in the field in the near future.

This paper provides an overview of some current and emerging physicochemical analytical challenges associated with these sophisticated drug systems and some of the technical advances needed in chromatographic systems to enable their design, development, and manufacture.

Earlier this summer, separation scientists from around the world made their way to San Diego for the HPLC 2022 conference. What was it like being back to an in-person liquid chromatography conference?

The effect of the mobile phase composition, organic alcohols, ion-pair reagents, and different bonding chemistries on mAb separations is discussed.

As two-dimensional liquid chromatography (2D-LC) becomes more widely used, system suitability tests (SSTs) become even more important.

With a new prototype, we explore how to improve the resolution power and usability of LC–MS instruments for routine analysis in pharmaceutical applications.

Although well known, the extent of adduct ion formation in LC–ESI-MS/MS, and the implications for quantitative analysis and analyte characterization are not fully appreciated. We explore this problem and explain the implications for reproducibility, quantitative and qualitative analyses, and DDA and DIA.

With the advent of ambient ionization and portable mass spectrometers, the ability to perform rapid, on-site analysis is fast becoming a reality. We review the critical recent developments enabling this capability as well as remaining challenges that must be tackled to enable widespread adoption.

Developing and building a talent base in macromolecular separations should be a priority for employers. Here’s why.

Using Earth as a figurative analogy, we explore the merits of flexibility when considering which liquid chromatography (LC) modality to use for any particular bioanalysis.

We explain several of the more frequent causes of bad peak shapes in liquid chromatography and provide tips on how to remedy them.

Knowing the variables that most affect the sample loading capacity in a porous layer open tubular (PLOT) column is vital to determining how much sample to inject.

We kick off a new article series that offers an insider’s view of the pharmaceutical industry, focusing on the role of separation science and separation scientists.

In this issue, we feature fundamental and innovative efforts from within the walls of some the leading companies in the separation science industry. The theme was inspired by the continuous output of essential separation tools and the seemingly endless streams of pertinent information that these companies provide the scientific community.

Sustainability concerns are renewing interest in SFC, often as a substitute for HPLC. With the broader application of SFC, we need a better understanding of selectivity in SFC, where stationary phase classification is not well established. This study assessed and measured the selectivity differences afforded by three prototype SFC phases.

Superficially porous particles (SPPs) are routinely used in analytical liquid chromatography (LC) columns, but they also provide benefits for preparative LC columns for use in pharmaceutical discovery laboratories and high-throughput environments. In this study, a preparative method using SPP-packed column had a 45% decrease in run time over a method using columns packed with fully porous particles, at standard flow.

A novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.

Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D-LC) separations. Here, two approaches are presented that can help navigate this challenge.

Our yearly report on liquid chromatography (LC) columns and accessories commercially released over the past year.

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.