Mass Spectrometry

This article examines the various effects of phospholipids in liquid chromatography tandem mass spectrometry (LC–MS-MS) analysis and demonstrates a new phospholipid-removal approach.

This instalment provides a brief overview of some multidimensional approaches targeted at intact protein analysis for life science applications, particularly for biopharmaceutical analysis.

This article reports on the isolation, purification, and characterization of phorbol esters in Jatropha curcas seeds.

Here, the process to build an accurate mass database is thoroughly described and applications are commented on.

Gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) techniques offer advantages in separating and confirming the identity of constituents in novel psychoactive substances.

Accurate-mass approaches offer a significant advance over nominal-mass approaches in the arena of qualitative analysis, and some of the analytical approaches can now be conducted in a relatively routine manner.

A study of colony collapse disorder in honey bees illustrates how mass spectrometry–based proteomics techniques can be used to to identify pathogens without any prior knowledge of what is contained in the sample.

A proof-of-concept application for detecting abused steroids in urine samples collected from an antidoping surveillance program

An update on the sample preparation and LC–MS-MS tools available for allergen detection, as well advantages of those techniques.

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS

A discussion of active pharmaceutical ingredient (API) selection, drug product development, and mass spectrometry instrumentation

A review highlighting the combination, or potential combination, of various separation methods with ambient ionization mass spectrometry

How to create a liquid chromatography–tandem mass spectrometry (LC–MS-MS) system using mass spectrometers, a high performance liquid chromatography (HPLC) binary pump system, and an autosampler

A breakthrough using a microfluidic interface to conduct sensitive time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis and study liquid surfaces in situ under vacuum conditions is described here.

A critical look into the future of imaging mass spectrometry is made by addressing some of the fundamental and technical challenges that still need to be overcome.

A method is illustrated for the simultaneous analysis of ethyl glucuronide and ethyl sulfate in human urine samples.

The combination of GC with time-of-flight (TOF)-MS proves to be a successful approach for the challenging analysis of persistent organic pollutants in complex matrices such as sediment and fish samples.

This study illustrates the versatility of a laser-induced acoustic desorption (LIAD) approach for volatilization of analytes in modern mass spectrometry.

High-definition screening by gas chromatography–mass spectrometry (GC–MS) is shown to be a viable option for the reliable identification of odorous compounds in pork.

Ion mobility–mass spectrometry (IM-MS) is outlined as a separations method, several examples of the utility of IM-MS for complex biological measurements are illustrated, and the implications of this approach for systems biology research are discussed.

Identifying unknown compounds continues to challenge analysts.

On the 50th anniversary of the invention of the laser, Michael Balogh examines its importance in MS practice.

In mass spectrometry, the electrospray ionization technique quickly earned its place among the primary analytical tools. Yet it is not always the best tool for the job.

With the advances in LC-MS in recent years, is there another practical option on the horizon for those who need the specificity of MS and can justify the narrow definition of a purpose-built system?

This month's installment addresses some elementary aspects of the mass spectrum, the tools employed by the experienced practitioner, and advances being made into identifying unknowns.