Mass Spectrometry

These case studies illustrate how the pharmaceutical industry utilizes mass spectrometry–based solutions to address product quality concerns, particularly when extremely low detection limits are required and when development resources are limited.

Even compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.

Non-proximate MS presents challenges for ion transmission and preventing loss of analyte signal. For continuous, direct MS analysis, there are four models for non-proximate MS that work best.

This study illustrates the importance of column selection during GC method development.

In recent years, top-down mass spectrometry has quickly advanced from academic rarity to (near-) routine. How did we get here? And what can we expect in the near future?

Recent developments in high-resolution mass spectrometry (HRMS) are aiding biopharmaceutical development and simplifying routine monitoring, with applications in areas like multi-attribute methods, coupling established purity methods with MS, and subunit analysis.

HS-SPME-GC–MS was combined with OPLS-DA data analysis to tentatively identify eight chemical markers to differentiate the geographical origins of cigar leaf samples.

Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

This 7-minute method enables easy analysis of bitter flavors in beer.

At ChromTalks, experienced speakers shared mistakes made during their careers.

Mary Ellen McNally knows how to solve complex and pressing problems in the industry.

Modern ion mobility–mass spectrometry (IM–MS) is a key separation technology for detailed molecular characterization studies and also as part of emerging data acquisition strategies for demanding small molecule and several applications. Here is what you need to know.

Analysis of extractables and leachables (E&L) from plastic packaging is of great importance for pharmaceutical product safety. Accurate and rapid identification of unknown compounds in E&L is often complex and challenging. To address this challenge, we demonstrate a quick method for oligomer determination using LC–QTOF-MS.

Supplementing short pulse lasers with laser postionization increases ionization yields for desorption and ablation of solid samples in mass spectrometry. Here, we give an overview of the mechanisms and technical requirements for molecular photoionization in femtosecond (fs) laser desorption postionization mass spectrometry (LDPI-MS).

Using ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.

Two-dimensional liquid chromatography (2D-LC) allows much greater resolution of peaks than is possible in a classical single dimensional separation. For the next development in separations, we employed 2D-LC in two highly orthogonal dimensions of separation with four mass spectrometers for detection, with parallel detection in each dimension. We have further broken ground by using three dimensions of separation with four mass spectrometers, using two parallel second dimensions.

Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.

Highlights of the new high-performance liquid chromatography, mass spectrometry, and chromatography data systems introduced over the past year.

We present our annual review of new products in gas chromatography, introduced between spring 2020 and spring 2021.

This article looks at the benefits of combining dynamic headspace sampling (DHS) with capillary GC–TOF-MS as a tool for untargeted analysis of aroma compounds in food and beverages. Applications for the analysis of strawberry yoghurt, chocolate, and red wine are described.

In the second part of this review of the current state of HIC, some practical considerations are explained, including method development, selection of the phase system, combined salt systems, and possibilities to combine HIC with other chromatographic modes.

LCGC Europe spoke to Stefan Van Leeuwen and Bjorn Berendsen from Wageningen Food Safety Research, The Netherlands, about a novel non-targeted approach to analyze PFASs using LC–HRMS with fragment ion flagging (FIF).

Rebecca Gowland and her colleagues in the Department of Archaeology at Durham University in the United Kingdom have tested, for the first time, the applicability of a new method of sex estimation utilizing enamel peptides from a sample of permanent and deciduous teeth at different stages of mineralization, from nonadults of unknown sex, including perinates, and using a minimally destructive acid etching procedure and subsequent nano liquid chromatography– tandem mass spectrometry. She spoke to us about her efforts.

Industrial laboratories have played a major role in innovation and commercialization of new technologies in energy and chemicals. In this special session, Joseph Powell, the Chief Scientist at Shell Oil Company, will present case studies in technology development in biofuels and new energies.

This Monday afternoon session brings together five young leaders in the field of mass spectrometry (MS) to discuss cutting-edge developments in imaging MS technologies. These presentations will be of interest to practitioners of metabolomics, proteomics, imaging, fundamental ion chemistry, and biomedical analyses, as well as the analytical community at large.