HPLC

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.

An undeniable effect of the COVID-19 pandemic is that we have learned how to adapt (quickly) to sudden changes.

Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.

QuEChERS has been updated to suit modern instrumentation. Now also “efficient and robust,” QuEChERSER is a “mega-method” that covers a wider polarity range.

We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.

Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.

Errors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.

Are we ready to let software and instruments take over the art of separations?

Here, a new automated active pharmaceutical ingredient (API) extraction platform is integrated with online liquid chromatography (LC) to assess drug product potency. The platform dissolves the tablet coating (if present), disintegrates the tablet, and disperses and solubilizes the tablet for analysis.

Deviations from the expected pressure in modern LC systems (too low, too high, or fluctuating) can be diagnosed and more quickly resolved using the streamlined troubleshooting practices shown here.

In this month’s blog, we provide information about the Subdivision on Chromatography and Separations Chemistry (SCSC) of the Analytical Division of the American Chemical Society (ACS), sharing our main goals and introducing our newly appointed executive board members.

Method validation processes will need to be refined to better suit forensic laboratories.

An improved LC–MS/MS method that can rapidly detect fipronil, an insecticide harmful to human health, is presented here for chicken eggs, feed, and soil.

What leads to an asymmetric peak shape? Physiochemical phenomena can help chromatographers identify whether the cause of asymmetry has a chemical or physical origin.

In this series of blog posts, I’m going to explore how the challenges of adopting methods from the literature, or from internal or external clients, can often be made easier, and more enjoyable, by taking time for some detective work prior to even entering the laboratory.

The UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.

With a new free simulator, educators and students can perform virtual HPLC experiments under isocratic or gradient conditions applicable to real instrumentation and method development.

As the field moves toward routine use of pressures well above 400 bar, the effect of pressure on retention should not be overlooked.

Understanding the fundamentals of extraction methodologies is critical, and can enable chromatographers to find smart solutions to tackle the complex composition of different types of samples.

Are the days numbered for long linear runs of univariate data in separation science?

Three-dimensional (3D) printing shows promise for creating stationary phases with tailored geometries. We present a method that overcomes limitations of other approaches, and can produce columns with a feature resolution of 10 μm.

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.

In a continuing series on peak shapes, we focus on potential physical causes of asymmetry, including column packing, changes in the packed particle bed, and accumulation of debris in the column.

Professor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.