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The Application Notebook-06-01-2011

The Application Notebook

Improving SFC Chiral Separations by Employing a New Chlorinated Polysaccharide Chiral Stationary Phase: The RegisPack CLA-1

June 01, 2011

Chiral

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As the number of chiral columns and chiral separations has grown, it has become difficult to organize the information for use in developing new methods. The success of chiral method development depends on the introduction of appropriate columns. The RegisPack? polysaccharide-based chiral stationary phase (CSP) is most successful in achieving a majority of chiral separations. However, there are cases in which this proven chiral selector leads to partial or no separation. Excellent separations using the newly introduced RegisPack? CLA-1 CSP have been obtained for a range of compounds, either by improving or complementing RegisPack's selectivity.

Aggregated Singletons for Automated Purification Workflow

June 01, 2011

Articles

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Aggregated singletons for automated purification (ASAP) is a purification workflow that provides singleton sample purification, registration, and delivery to the materials management department as 30 mM dimethyl sulfoxide solutions for biological screening.

Cleanup of Baby Food Samples Using Gel Permeation Chromatography (GPC)

June 01, 2011

Food and Beverage

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The Food Safety Modernization Act, which was recently signed into law, is the country's first major food legislation since 1938, and increases the FDA's authority over the U.S. food supply. These new regulations require that foods, including baby food, be tested for pesticide residues.

Chemical Composition Analysis of Polyolefins by Multiple Detection GPC-IR5

June 01, 2011

Polymer

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Polyolefin (PO) is the largest volume industrial polymer in the world for making a wide range of commercial products that touch nearly every aspect of our daily lives, such as automobile parts, pipes, packaging films, household bottles, baby diapers, and so on.

Separation of Quaternary Ammonium Compounds Using a Bonded Polymeric Zwitterionic Stationary Phase

June 01, 2011

Environmental

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Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry. The impact of ionic strength and organic content in the mobile phase is discussed.

BioPharma Compass: A Fully Automated Solution for Characterization and QC of Intact and Digested Proteins

June 01, 2011

Biological

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BioPharma Compass? is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA. This push button solution assists nonspecialist operators to generate high quality, accurate data for automatic comparison with laboratory reference standards. Automated, visual reports are then generated for each sample and important information regarding a products purity and identity can be observed at a glance. In this application note, we will apply the BioPharma Compass workflow to the QC characterization of two proteins including intact IgG1 and digested transferrin.

Resistive Glass Inlet Tubes Increase Ion Throughput

June 01, 2011

General

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Resistive glass tubes and plates are designed to guide ions by generating a uniform electric field. PHOTONIS resistive glass products are composed of a proprietary lead silicate glass that has been specially processed to create a resistive layer at the surface. The resistivity can be varied over several orders of magnitude to suit the specific application.

Method 525.2 Update: Analyte Recoveries with New CCL3 Compounds

June 01, 2011

Environmental

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EPA is updating method 525.2. The CCL3 includes 104 analytes. UCT has evaluated the recoveries of these compounds, including the current list, using proposed changes to the method with the C18-based UCT ENVIRO-CLEAN? 83 mL Extraction Cartridge ECUNI525.

Isolation of Benzylideneacetophenone from a Crude Reaction Mixture

June 01, 2011

General

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Benzylideneacetophenone forms the central core for a variety of important biological compounds, known collectively as chalcones. Many of these compounds have significant medicinal value as they demonstrate antibacterial, antifungal, antitumor, and anti-inflammatory properties.

Novel C18 Stationary Phase Provides Exceptional Results for Metabolite Profiling

June 01, 2011

Biological

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Metabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.

Self-Aggregation of Gelatin

June 01, 2011

Biological

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Gelatin is the denatured form of collagen, the main component of skin, bone, and connective tissues of animals. Its special properties are a sharp sol-gel transition, low sol viscosity, and high effectiveness as a protective colloid.

Extraction of Sub-Nanogram Levels of Catecholamines from Human Plasma Using EVOLUTE? WCX

June 01, 2011

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A new, efficient, effective, and sensitive sample preparation application for the extraction of the catecholamines epinephrine, norepinephrine, and dopamine from human plasma at significantly low levels using EVOLUTE WCX, a resin-based mixed-mode sorbent with an optimized combination of nonpolar (hydrophobic) and weak cation exchange interactions.

Rapid and Streamlined Screening of Barbiturates

June 01, 2011

General

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Barbiturates are a class of antidepressants whose abuse and addiction by recreational users has become a widespread problem (www.nlm.nih.gov). In our work we strived to streamline the barbiturate screening process to provide a fast, cost-effective, and reproducible method from start to finish for forensic labs who are involved in high-throughput processing.

Determination of Explosives Using Fast and High Resolution Liquid Chromatography with the Agilent 1290 Infinity LC System

June 01, 2011

Environmental

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This application note describes the method for detection of nanogram levels of explosive constituents in seawater samples (e.g., after detonation of unexploded ordnance devices). The improved chromatographic method was developed using the Agilent 1290 Infinity LC Method Development system.

Enantiomeric Separation of Proton Pump Inhibitors Using Polysaccharide-Based Chiral Stationary Phases in Reversed-Phase HPLC Conditions

June 01, 2011

Pharmaceutical

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The chiral analysis and purification of proton pump inhibitors (PPIs) has become a popular topic as more of these drugs fall out of patent protection each year. Due to their wide range of enantiomeric selectivity, a set of polysaccharide-based chiral stationary phases (CSPs) was screened to identify methods for the successful enantioseparation of four benzimidazoles; rabeprazole, lansoprazole, omeprazole, and pantoprazole.

Fast Determination of Catechins in Tea

June 01, 2011

Food and Beverage

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Tea, one of the most consumed non-alcoholic drinks worldwide, contains catechins that are powerful antioxidants thought to provide many health benefits, including reduction of cholesterol and obesity, and protection against cardiovascular disease and cancer.

A New Path to High-Resolution HPLC–TOF-MS — Survey, Targeted, and Trace Analysis Applications of TOF-MS in the Analysis of Complex Biochemical Matrices

June 01, 2011

Articles

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High performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.

Improved Analysis of Preservatives in Cosmetics Using a Unique C18 Core-Shell Phase

June 01, 2011

General

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This study evaluates the performance of a C18 core-shell phase that incorporates a C18 ligand with iso-butyl side chains. The Kinetex? XB-C18 HPLC/ UHPLC column delivers a fast and effective separation of several common preservatives in cosmetics.

Advantages of Multi-Pesticide Screening by GC–MS

June 01, 2011

Environmental

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Due to the increasing number of toxic pesticides being used throughout the world, a robust method for positively identifying low levels of multiple classes of pesticides is required. A Zebron? ZB-MultiResidue? column is used with GC–MS to identify over 100 common pesticides.

Analyzing Testosterone in Human Serum by UHPLC Using High Efficiency Kinetex 1.7 m C18 Core-Shell Columns

June 01, 2011

Biological

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Testosterone was extracted from human serum by strong anion exchange polymeric SPE and analyzed using a Kinetex C18, 30 ? 2.1 mm, 1.7 ?m column and positive polarity ESI LC–MS-MS system. Kinetex sub-2 ?m core-shell technology offers higher efficiencies than traditional sub-2 ?m columns, producing greater chromatographic resolution, sensitivity, and higher peak capacities.

Reducing Cycle Time for Analysis of 1,4-Dioxane Using an Automated Purge and Trap Sample Prep System

June 01, 2011

Environmental

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With advances in instrumentation and without regulatory method constraints, 1,4-dioxane can be detected at the part-per-billion (ppb) level, despite its poor purge efficiency. This application will manipulate purge and trap, as well as GC–MS parameters, to create a more efficient method to reduce cycle times. This study will utilize a Teledyne Tekmar Atomx Automated Sample Prep System in conjunction with an Agilent 7890/5975 GC–MS. A linear calibration and method detection limits (MDLs) for 1,4-dioxane will be established using this method.

Fast and Accurate LC–MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns

June 01, 2011

General

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Vitamin D deficiency has become a topic of interest in recent publications (1–3). Vitamin D is present in two forms, Vitamin D3 and Vitamin D2. D3 is produced after ultraviolet light-stimulated conversion of 7-dehydrocholesterol in the skin (3).

LC Analysis of Aminoglycoside Antibiotics Kanamycin and Amikacin

June 01, 2011

Pharmaceutical

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Kanamycin and amikacin are aminoglycoside antibiotics used to treat serious bacterial infections. Amikacin is used for infections resistant to other aminoglycosides because it is less susceptible to enzymatic reactions.

Vol 29 No 6 LCGC North America June 2011 Application Notebook PDF

June 01, 2011

Issue PDF

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Click the title above to open the LCGC North America June 2011 Application Notebook, Vol 29 No s6, in an interactive PDF format.