The Application Notebook-06-01-2011

Hydrophilic interaction chromatography (HILIC) has become a popularly used separation mode for carbohydrate HPLC method. There are wide variations in HILIC stationary phase.

As the number of chiral columns and chiral separations has grown, it has become difficult to organize the information for use in developing new methods. The success of chiral method development depends on the introduction of appropriate columns. The RegisPack? polysaccharide-based chiral stationary phase (CSP) is most successful in achieving a majority of chiral separations. However, there are cases in which this proven chiral selector leads to partial or no separation. Excellent separations using the newly introduced RegisPack? CLA-1 CSP have been obtained for a range of compounds, either by improving or complementing RegisPack's selectivity.

The Application Notebook
Biological

June 01, 2011

Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications. The HES's circulation time in the blood depends strongly on its molar mass distributions.

The Application Notebook

Aggregated singletons for automated purification (ASAP) is a purification workflow that provides singleton sample purification, registration, and delivery to the materials management department as 30 mM dimethyl sulfoxide solutions for biological screening.

Determination of pharmaceuticals in biological matrices is commonly employed in ADME (DMPK), clinical, and forensic analysis. The main techniques used for drug monitoring and analysis are immunoassays, LC, and GC methods.

The Application Notebook

The Food Safety Modernization Act, which was recently signed into law, is the country's first major food legislation since 1938, and increases the FDA's authority over the U.S. food supply. These new regulations require that foods, including baby food, be tested for pesticide residues.

Polyolefin (PO) is the largest volume industrial polymer in the world for making a wide range of commercial products that touch nearly every aspect of our daily lives, such as automobile parts, pipes, packaging films, household bottles, baby diapers, and so on.

The presented ion chromatographic method is used for the simultaneous determination of HF, HNO3, H2SO4, short-chain organic acids and H2SiF6 in acidic texturing baths that are used in the wet chemical etching process of solar cell production.

Synthetic cannabis products have garnered a great deal of media attention recently. Countless products are being sold at smoke shops, convenience stores, and online sites.

Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry. The impact of ionic strength and organic content in the mobile phase is discussed.

BioPharma Compass? is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA. This push button solution assists nonspecialist operators to generate high quality, accurate data for automatic comparison with laboratory reference standards. Automated, visual reports are then generated for each sample and important information regarding a products purity and identity can be observed at a glance. In this application note, we will apply the BioPharma Compass workflow to the QC characterization of two proteins including intact IgG1 and digested transferrin.

The Application Notebook

Resistive glass tubes and plates are designed to guide ions by generating a uniform electric field. PHOTONIS resistive glass products are composed of a proprietary lead silicate glass that has been specially processed to create a resistive layer at the surface. The resistivity can be varied over several orders of magnitude to suit the specific application.

The Application Notebook

EPA is updating method 525.2. The CCL3 includes 104 analytes. UCT has evaluated the recoveries of these compounds, including the current list, using proposed changes to the method with the C18-based UCT ENVIRO-CLEAN? 83 mL Extraction Cartridge ECUNI525.

Fast, flexible platforms for peptide quantification are needed, particularly for a discovery setting. This type of methodology would be especially advantageous in the case of amyloid beta (a?) peptides.

The Application Notebook

Benzylideneacetophenone forms the central core for a variety of important biological compounds, known collectively as chalcones. Many of these compounds have significant medicinal value as they demonstrate antibacterial, antifungal, antitumor, and anti-inflammatory properties.

Metabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.

The Application Notebook
Biological

June 01, 2011

Gelatin is the denatured form of collagen, the main component of skin, bone, and connective tissues of animals. Its special properties are a sharp sol-gel transition, low sol viscosity, and high effectiveness as a protective colloid.

The Application Notebook

This study demonstrates a faster method of analysis when coupling a simplified and effective SPE procedure with a core-shell HPLC column and LC–MS-MS detection.

The Application Notebook

One of the biggest debates in clinical medicine currently revolves around understanding the proper levels of vitamin D in patients' plasma.

Biogenic amines are present in protein-rich foods such as fish, meat, and milk. Their concentration increases during food decomposition.

Hydroxymethylfurfural (HMF), an organic compound containing aldehyde and alcohol functional groups, is naturally found in very low amounts in sugar-containing foods.

A new, efficient, effective, and sensitive sample preparation application for the extraction of the catecholamines epinephrine, norepinephrine, and dopamine from human plasma at significantly low levels using EVOLUTE WCX, a resin-based mixed-mode sorbent with an optimized combination of nonpolar (hydrophobic) and weak cation exchange interactions.

The Application Notebook

Barbiturates are a class of antidepressants whose abuse and addiction by recreational users has become a widespread problem (www.nlm.nih.gov). In our work we strived to streamline the barbiturate screening process to provide a fast, cost-effective, and reproducible method from start to finish for forensic labs who are involved in high-throughput processing.

International regulations on maximum residue levels (MRLs) of pesticides in food cover hundreds of individual contaminants at the 10 ppb or below range. The analysis of citrus oil for pesticide contamination holds specific challenges.

The Application Notebook

Endothall is a widely used herbicide for both terrestrial and aquatic weeds. Exposure to endothall in excess of the maximum contamination level (MCL) can cause illness. Endothall is regulated by the U.S.

This application note describes the method for detection of nanogram levels of explosive constituents in seawater samples (e.g., after detonation of unexploded ordnance devices). The improved chromatographic method was developed using the Agilent 1290 Infinity LC Method Development system.

The chiral analysis and purification of proton pump inhibitors (PPIs) has become a popular topic as more of these drugs fall out of patent protection each year. Due to their wide range of enantiomeric selectivity, a set of polysaccharide-based chiral stationary phases (CSPs) was screened to identify methods for the successful enantioseparation of four benzimidazoles; rabeprazole, lansoprazole, omeprazole, and pantoprazole.

The Application Notebook

The chemicals 2- and 4-methylimidazole (2-MI and 4-MI) are by-products produced during the manufacturing of caramel coloring ingredients used to darken food products such as carbonated beverages and soy sauce.

The Application Notebook

Anthocyanins are a sub-class of naturally electron deficient and powerful antioxidants called flavonoids that are responsible for the red, orange, and blue coloration in fruits and flowers.

The Application Notebook
Food and Beverage

June 01, 2011

Tea, one of the most consumed non-alcoholic drinks worldwide, contains catechins that are powerful antioxidants thought to provide many health benefits, including reduction of cholesterol and obesity, and protection against cardiovascular disease and cancer.